Template Switch Oligo

Template Switch Oligo - A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. However, if a large fraction of the library contains the. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. The resulting cdna can be amplified by pcr or serve as.

(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. However, if a large fraction of the library contains the. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. We recommend a tso with rgrgrg at the 3′ end. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. The resulting cdna can be amplified by pcr or serve as.

Fig. S12 Schematic representation of the tested oligodT / TSO

However, if a large fraction of the library contains a portion. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. Template switching oligonucleotide and capturing oligo (dt) are.

Tuning 5’ to internal read proportions and template switching oligo PCR

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. We recommend a tso with rgrgrg at the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. However, if a large fraction of the library contains a portion.

Template Switching Oligo

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at.

Template Switch Oligo

However, if a large fraction of the library contains a portion. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. A small fraction of.

Template Switch Oligo

However, if a large fraction of the library contains a portion. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains the. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the.

Template switching oligos (TS oligos, TSOs) for cDNA library

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. A small fraction.

NanoCAGE A HighResolution Technique to Discover and Interrogate Cell

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of single cell 3' libraries are expected to contain the template.

Template switch and twin priming. (A) Steps describing template

Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. The resulting cdna can be amplified by pcr or serve as. However, if a large fraction of the library contains a portion. We recommend a tso with rgrgrg at the 3′ end. In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains the. However, if a large fraction of the library contains a portion. Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the.

(A) First strand cDNA is initiated by priming with an oligo dT primer

Use this set of oligonucleotides for template switching cdna synthesis and preamplification. A small fraction of visium libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains a portion. (step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. We recommend a tso.

The Resulting Cdna Can Be Amplified By Pcr Or Serve As.

In conjunction with a template switching oligo (tso), cdna is synthesized with a known sequence of choice attached to the 3′ end. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. Template switching oligonucleotide and capturing oligo (dt) are used to incorporate adaptor. We recommend a tso with rgrgrg at the 3′ end.

A Small Fraction Of Visium Libraries Are Expected To Contain The Template Switching Oligo (Tso) At The Beginning Of Read 2.

(step 2) mmlv reverse transcriptase adds deoxycytosines to the cdna 3' end. However, if a large fraction of the library contains a portion. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. Use this set of oligonucleotides for template switching cdna synthesis and preamplification.