Template Switching Oligo

Template Switching Oligo - The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. The tso adds a common 5'. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains the. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The mix contains a unique rt. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent.

The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. We recommend a tso with rgrgrg at the 3′ end. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. However, if a large fraction of the library contains the. The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The mix contains a unique rt.

Template Switch Oligo

To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching.

Fig. S12 Schematic representation of the tested oligodT / TSO

However, if a large fraction of the library contains the. We recommend a tso with rgrgrg at the 3′ end. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added.

(A) First strand cDNA is initiated by priming with an oligo dT primer

A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. The tso adds a common 5'. In general, different tsos can be used including.

(A) The TS mechanism is used for first strand cDNA synthesis. First, an

The tso adds a common 5'. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The mix contains a unique rt. The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. However, if a large fraction of.

Tuning 5’ to internal read proportions and template switching oligo PCR

The tso (template switch oligo) is an oligo that hybridizes to untemplated c nucleotides added by the reverse transcriptase during reverse transcription. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. However, if a large fraction of the library contains the. Template switching oligo and capturing oligo (dt) primer.

What Is A Template Switching Oligonucleotide

The tso adds a common 5'. The mix contains a unique rt. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. However, if a large fraction of the library contains the. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages.

AccelerRT® 5G Template Switching RT Enzyme Mix GeneCopoeia™

The tso adds a common 5'. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. Template switching oligo and capturing oligo (dt) primer are.

Model of cDNA synthesis that occurs in the absence (A) and presence (B

The mix contains a unique rt. The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. However, if a large fraction of the library contains the. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse. To.

Template Switching Oligo

The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. We recommend a tso with rgrgrg at the 3′ end. The mix contains a unique rt. Template switching.

Template switching oligos (TS oligos, TSOs) for cDNA library

The tso adds a common 5'. However, if a large fraction of the library contains the. A small fraction of single cell 3' libraries are expected to contain the template switching oligo (tso) at the beginning of read 2. In general, different tsos can be used including tsos with 5′ modifications, such as biotin, isomeric bases or abasic spacers. The.

In General, Different Tsos Can Be Used Including Tsos With 5′ Modifications, Such As Biotin, Isomeric Bases Or Abasic Spacers.

The template switching rt enzyme mix and accompanying reaction buffer enable efficient template switching activity in a reverse transcription reaction. The tso adds a common 5'. We recommend a tso with rgrgrg at the 3′ end. However, if a large fraction of the library contains the.

The Tso (Template Switch Oligo) Is An Oligo That Hybridizes To Untemplated C Nucleotides Added By The Reverse Transcriptase During Reverse Transcription.

The mix contains a unique rt. Template switching oligo and capturing oligo (dt) primer are designed for use in template switching reverse transcription reactions, while preamplification primer is used in subsequent. To maximize template switching eficiency, the master mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (tdt) activity of. The false positive clones were tandem repeats of different lengths of the smart ivtm oligonucleotide and originated through repeated template switching during reverse.